WebEnter the email address you signed up with and we'll email you a reset link. WebJan 1, 2016 · The total volume was adjusted to 1 ml in each condition. The amount of HMGB1 and ATP in the culture medium was measured using an HMGB1 enzyme-linked immunosorbent assay II kit (SHINO-TEST, Tokyo, Japan) and a luciferin-based ENLITEN ATP assay (Promega, Madison, WI), respectively, according to each manufacturer’s …

A Veritas™ Microplate Luminometer Method for Promega …

WebNov 25, 2015 · Purified or immobilized Luc was mixed with luciferin, Mg 2+, K + and ATP as indicated in individual experiments and activity was monitored by means of light emission as detected by a luminometer (GloMax, Promega, Madison, WI), where the luminescence signal was integrated for 3 seconds every minute for up to 50 minutes, as indicated per … WebENLITEN® ATP Assay System 100 assays The ENLITEN® ATP Assay System can be used to measure ATP levels for the indirect detection of biocontamination on food processing surfaces, in cosmetics and beverages or to assay for enzymes that degrade ATP and to quantitate ATP in biological fluids. how many sildenafil can you safely take

Astrocytes contribute to pain gating in the spinal cord

WebDec 15, 2024 · Supernatant was collected and analyzed using an ENLITEN ATP assay (Promega, Madison, WI, USA) and HMGB1 ELISA kit II (Shino-Test, Kanagawa, Japan). Western blotting. Cells were seeded in a 100-mm dish at a density of 1 × 10 6 cells/dish 24 h before viral infection. Murine and human PDAC cells were infected with OBP-301, Ad … Webq Promega ENLITEN ® ATP Assay System Bioluminescence Detection Kit for ATP (P/N FF2000) containing one vial rLuciferase/Luciferin (rL/L) Reagent, 12 mL Reconstitution Buffer, one vial ATP Standard (1x10-7 M), 25 mL ATP-Free Water B ... Madison, WI 53711 USA. Title: 997_9307_1_0.doc WebGloMax® 20/20 Luminometer and the ENLITEN ATP Assay System. 10 μL of ATP Standard diluted in HEPES buffer was added to a test tube containing 100 μL rL/L Reagent. When designing your ATP assay with the ENLITEN Kit, it is important to remember several key aspects of the luciferase reaction. First, the rL/L reacts optimally at a pH of 7.73 how did monkeypox get to usa 2022