TīmeklisSpecifically, outer membrane fusion is analyzed by mixing mitochondria isolated from cells expressing an outer membrane-targeted green fluorescent protein (om-GFP) … TīmeklisDsRed surprisingly takes days at room temperature to reach full red fluorescence. At room temperature, a sample of purified protein initially shows a major component of green fluorescence (ex/em 475/499 nm), which peaks in intensity around 7 h and decreases to nearly zero over 2 days. Meanwhile the red fluorescence reaches half …
DsRed和DsRed2红色荧光蛋白激发光与发射光波长 - LUYOR
Tīmeklis2013. gada 2. febr. · To visualize mitochondrial morphology and inheritance, the indicated strains were transformed with pVT100U-dsRED. Before imaging, the strains were grown in SC−Ura+Dex, and calcofluor was added to the medium at a final concentration of 100 μg/mL for 5 min before imaging. TīmeklisdsRed, created by replacing GFP in pVT100U-mtGFP with dsRed2 (3), a gift from Dr. B. Glick, University of Chicago, Chicago, IL, USA). For outer membrane fusion reactions, mitochondria were harvested from W303 cells transformed with two episomal plasmids containing m-dsRed and outer membrane targeted GFP (om-GFP, downscaling definition
Enhancer Trapping With a Red Fluorescent Protein Reporter inDrosophila
Tīmeklis2013. gada 22. janv. · To visualize mitochondrial morphology and inheritance, the indicated strains were transformed with pVT100U-dsRED. Before imaging, the strains were grown in SC−Ura+Dex, and calcofluor was added to the medium at a final concentration of 100 μg/mL for 5 min before imaging. TīmeklisTo construct pYX142-dsRed, GFP in pYX142-mtGFP was replaced with dsRED using standard cloning techniques (24). Yeast cytological analysis To visualize mitochondria and ER in WT cells, W303 GFP-HDEL was transformed with pVT100U-dsRED. To visualize mitochondria and ER in the absence of Rtns/Yop1, NDY257 was … Tīmeklis(A-C) fis1∆ mutants expressing matrix-targeted DsRed and outer-membranetargeted OM45-GFP were grown in PMGal to mid-log phase. After embedding in 1% agarose, live cells were imaged by ... downscaling model